Dr. Joseph Gabriele, Ph.D
Assistant Professor, Department of Psychiatry and Behavioural Neuroscience
Joseph Gabriele received his B.Sc degree from the University of Toronto, while his M.Sc. and Ph.D. degrees were received from McMaster University. During Joseph’s Ph.D. training he worked under the supervision of Dr. R.K. Mishra, focusing on molecular neuroscience and its implications in schizophrenia. He has received numerous awards including: a Canadian Institutes of Health Research (CIHR) studentship award in Pharmacology during his Masters degree; an NSERC Canada Graduate Scholarship during his Ph.D. training; an Ontario Mental Health Foundation Postdoctoral Fellowship and more recently, the International Congress on Schizophrenia Research, Young Investigator Award and Hamilton Health Sciences, New Investigator Award. Specifically, his research involved the cloning and characterization of a catecholamine-regulated protein in humans, which is specific to the central nervous system and blood. Further investigation revealed that this human CRP40 protein is also expressed in platelets and lymphocytes of human blood at both the mRNA and protein levels. Preliminary data has shown a significant reduction of human CRP40 mRNA expression in white blood cells of schizophrenia subjects relative to age-matched healthy controls.
Training at McMaster involved the use of many molecular biology techniques including:
- Northern and western immunoblotting
- Antisense and SiRNA experiments in rat models
- Microsurgeries using stereotaxic equipment
- Immunohistochemistry and immunocytochemistry
- Real-Time PCR
- Epigenetic analysis (DNA methylation)
In 2006 during his postdoctoral training, Joseph trained in a more clinical environment at Queen’s University, Kingston, Ontario under the direction of psychiatrist and World Health Organization (WHO) professor, Dr. A.N. Singh, in a project involving the metabolic side effects of antipsychotic medications in schizophrenia subjects. During this period, Joseph has also learned and performed numerous experiments using epigenetic protocols and with the encouragement of Dr. J. Bienenstock and Dr. B Christensen has begun collaboration with Dr. A. Petronis of the University of Toronto. Dr. Gabriele has recently been appointed as an Assistant Professor at McMaster University in the Department of Psychiatry and Behavioural Neurosciences, and his future goal is to be a translational researcher bridging basic scientists and clinicians, specifically in schizophrenia research. Joseph and Dr. Mishra are presently funded by the CIHR and Ontario Mental Health Foundation.
- Gabriele J, Rajaram M, Zhang B, Sharma S, and Mishra RK. (2002). Modulation of a 40-kDa catecholamine-regulated protein following D-amphetamine treatment in discrete brain regions. Eur J Pharmacol 453, 13.
- Zhang B, Van Adel BA, Gabriele J, Duong M, Henry P, Ball AK, and Mishra RK. (2002). Expression of the 40 kDa catecholamine regulated protein in the normal and injured rat retina. J Chem Neuroanat 24, 41-48.
- Gabriele J, Culver K, Sharma S, Zhang B, Szechtman H, and Mishra RK. (2003). Asymmetric modulation of a catecholamine regulated protein in the rat brain, following quinpirole treatment. Synapse, 49: 261-269.
- Sharma S, Paladino P, Gabriele J, Saeedi H, Henry P, Chang M, Mishra RK, and Johnson RL. (2003). Pro-Glycinamide and its peptidomimetic, PAOPA, attenuate haloperidol induced vacuous chewing movements in rat: A model of human tardive dyskinesia. Peptides, 24(2): 313-319.
- Gabriele J, Chong V, Pontoriero G, and Mishra RK. (2005). Decreased expression of a 40kDa catecholamine regulated protein in the ventral striatum of schizophrenic brain specimens from the Stanley Neuropathology Consortium. Schizophrenia Res, 74, 111-119.
- Sondri S, Carellano JM, Chong VA, Rogoza RM, Skoblenick KJ, Dyck BA, Gabriele J, Thomas N, Pristupa ZP, Singh AN, MacCrimmon D, Voruganti P, Foster J, and Mishra RK (2005). cDNA array reveals increased expression of glucose-dependent insulinotropic polypeptide following chronic clozapine treatment: Role in atypical antipsychotic drug-induced adverse metabolic effects. Pharmacogenomics J. Mar-Apr;6(2):131-40.
- Skoblenick KJ, Castellano JM, Rogoza RM, Dyck BA, Thomas N, Marriot J, Gabriele JP, Chong VZ and Mishra RK (2006). Translocation of apoptosis-inducing factor in the human and rat striatum following protracted haloperidol, but not clozapine treatment. Apoptosis. May;11(5):663-72.
- Gabriele J, Ross G, Nair V, and Mishra RK (2008) Role of catecholamine regulated protein (CRP40) in dopamine function. Life Sciences Mini Review, (accepted).
- Gabriele J, Thomas N, N-Marandi S, Mishra RK (2007). Differential modulation of a catecholamine regulated protein 40kDa in the core and shell sub-compartments of the nucleus accumbens following chronic quinpirole and haloperidol administration in the rat. Synapse,61:835-842.
- Gabriele J, Thomas N, Thomson C, Skoblenick K, Pristupa Z and Mishra RK (2008). Cloning, characterization and functional studies of a human 40kDa catecholamine regulated protein (Submitted to Cell Stress and chaperones)
- Gabriele JP, Thomas N, Mahadevan G, and Mishra RK (2008). Amount of antipsychotic drug treatment intake regulates mRNA expression of human 40 kDa catecholamine-regulated protein in the prefrontal cortex of schizophrenic and bipolar disorder specimens from the Stanley Foundation Neuropathology Consortium. (To be submitted to Schizophrenia Research March, 2009)
- Caixin Su , Nada Elfeki , Patrizia Ballerini , Iolanda D'Alimonte , Christian Bau , Renata Ciccarelli , Francesco Caciagli , Joseph Gabriele , Shucui Jiang (2009).Guanosine improves motor behavior, reduces apoptosis and stimulates neurogenesis in rats with parkinsonism (J Neurosci Res. 81:617-625.
- Gabriele J, Giuseppe Pontoriero, Thomas N, Thomson C, Skoblenick K, Pristupa Z and Mishra RK (2008). Cloning, characterization and functional studies of a human 40kDa catecholamine regulated protein (Cell Stress and Chaperones, accepted)